A fabrication method of networked semi-occlusive microfluidic was reported.This method uses femtosecond laser direct writing(FLDW) followed by chemical etching and covering a thin layer of polydimethylsiloxane(PDMS) film on fused silica glass substrate.The microfluidic channel formation mechanism is identified as substrate material modification caused by femtosecond laser irradiation,and modified areas can increase the solubility of hydrofluoric(HF) acid solution.The morphologies of networked microfluidic were characterized by using optical microscope and scanning electronic microscope.Furthermore,in order to test flowing characteristics of the microfluidic,the experiment of liquid injection was carried out.