The noninvasive measurement of human blood glucose is achieved with N IR diffusion reflectance spectrum technology. The wristN IR diffusion reflectance spectrum of six healthy volunteers is collected usingNexus2870 and its N IR Fiberport SmartAccessory. The test is imp lemented with the changing of the blood glucose concentration. The calibration model is set up using PLS method with the smoothing, baseline correct and second derivatives p retreatment spectrum in 7500～8500cm- 1 region for single volunteer and the combination of them. When the spectrum is got, the actual blood glucose value of every spectrum samp le is demarcated using ultraviolet spectrophotometer. The relativity between the calibration value and the actual one for single volunteer is better than that of the combination, and the correlative coefficient is up to 0. 99980. RMSEC are less than 0. 364, and the error is between - 0. 8～0. 8mmol/ l. Some samp les that are chosen op tionally don’t participate in setting up calibration model, and they are p redicted by themselvesmodel and other’s model respectively. The result of self-model p rediction is better than that of other volunteer’smodel, and the error of the p rediction is less than≤0. 89544mmol/ l.