Raman spectrum of solid DNA induced byβ2ray、γ2ray have been obtained by Raman spectroscopy. The result shows :after inducing byβ-ray、γ-ray ,the frequencies of adenine line at 1304cm- 1and 1340cm- 1 have a little changes ; the intensity of 786cm- 1 belonging to thymine and cytosine ,884cm- 1belonging to deoxyribose-phosphate and 1010cm- 1 belong- ing to C2O stretch vibration decrease by different degree ;1068cm- 1 belonging to C - O stretch vibration and 1468cm- 1 belonging to deoxyribose are disappeared.By analying we make out ;Afterβ-ray、γ-ray radiation ,the vibrations of deoxyribose , deoxyribose2phosphate and C2O stretch vibration will decrease in different degree ;the changes take place in T and A;the in teraction of base2base which perpendicular with DNA will be destroyed.