Fluorescent characteristic of dye laser materials including Rhodamine 6G, vulcanized Rhodamine B and Rhodamine 101, pumped by highpower laser was experimentally studied. Experiments were taken under various conditions such as: different solution density, different temperature etc. Experiment results show that for each kind of the materials, the optimized solution density corresponding to the largest fluorescent intensity, is influenced by the temperature of the dye solution to some extent. For vulcanized Rhodamine B and Rhodamine 101 the value of the optimized solution decreases as the temperature decreases, while for Rhodamine 6G the value does not change as the temperature changes. Theoretic analysis of these phenomenon was done in the end of the article.