The Raman spectra of red-cell from the normal blood and the malignant osteoma blood was given. For the malignant osteoma,lines of 1001.2,1228.8,1398.7,1372.8cm-1 were red shifted 2~4cm-1.It supported that the conformation of the base clusters were changed punily. The ratio of relative intensity of lines based on 1586cm-1 in red-cell were minished in 1505,1228,1087,1001 and 747cm-1,which showed the contents of heme,phenylalanine and deoxyribose in red-cell from the malignant osteoma were reduced. According to the statistic analysis,the changes of I1586/1228,I1586/1001 and I1586/1169 might provide a method for diagnosing malignant osteoma.