Based on the virtual instrument technology,the output video signals of readout circuit were acquired by the virtual instrument system constructed using NI PCI-6115 acquisition card.Driving signals were used as acquisition system′ control signals to control the start ,the end and the sample clock of acquisition.The design flows of acquisition program and the operation of front panel were presented in details.Then,acquisition experiments of the output signals of two chips developed in lab were taken out.The feasibility of single channel′ acquisition and multichannel acquisition were validated respectively.And it was approved that this acquisition system can acquire both single-channel and mutichannel infrared video signals with different pixel number.