An infrared image acquisition and non-uniformity correction system based on DSP and USB2.0 is designed to acquire real-time IR image of high quality.The original images detected by the IRFPA are collected in real time with EZ-USB FX2 as the USB2.0 interface chip.In addition,high-precision non-uniformity correction and contrast enhancement are achieved by high-performance floating processor TMS320C6713B so as to display the correction result on the monitor in real time.Data format conversion and timing control during acquisition process are implemented by FPGA,which improves the flexibility and reliability of the system.The experiment results indicate that this system can satisfy the requirements of real-time acquisition and high accuracy.