It is hard to differentiate the same species of wild growing mushrooms from different areas by macromorphological features.In this paper,Fourier transform infrared spectroscopy(FTIR)combined with hierarchical cluster analysis was used to identify 63 samples of Boletus speciosus from five different areas.The original characteristic infrared spectras of the mushrooms are very similar,with small differences in the range of 1800～1000 cm-1.Hierarchical cluster analysis was performed using the first derivative and second derivative spectral range 1800～1000 cm-1.The first derivative spectra give satisfactory results.All Boletus speciosus samples were divided into 5 classes with a classification accuracy of 90.5％.It is proved that FTIR spectroscopy combined with hierarchical cluster analysis could be used to discriminate wild growing mushrooms at species level.