To study the bioeffects of HPM on animal photoreceptor proteins structure and explore the HPM injury molecular mechanism,FT-IR microspectroscopy is applied to observe the change of biomembranes proteins structure in the outer segments.The results by FT-IR reveals that there are significant spectral differences of the absorption values for amideⅠband and the α-helix conformation decreases along with the increase of random coil and β-sheets structures after HPM exposure.It comes to the conclusion that the secondary structure of retinal membrane proteins has changed since the HPM irradiation,which may lead to the loss of proteins function.