The objective of the study is to compare the temperature characteristics of sub-health of neck fatigue and cervical spondylosis with infrared thermo-graphy.According to the results of a questionnaire survey and physical examination,the subjects are divided into health group，sub-health group and ailing group.Collecting each group′s infrared thermal images and using statistical analysis software to analyze the temperature difference effect,temperature difference areas,temperature difference ratio.It turns out that both sub-health group and ailing group show temperature rise.The ailing group′s temperature is significantly higher than that of the sub-health group.The average temperature，temperature difference ratio of the sub-health group,ailing group and health group have statistical significance(P<0.05).The average temperature,temperature difference ratio of the sub-health group and ailing group have also statistical significance(P<0.05).It comes to the conclusion that infrared thermo-graphy of the temperature effect，temperature region，temperature difference ratio can help in diagnosis of sub-health of neck fatigue and cervical spondylosis.