The palm vein image acquisition is difficult in palm vein pattern recognition.A multi-wavelength palm vein image acquisition system is proposed in this paper,which is low cost and can image the whole palm.A total of 2500 palm vein images are acquired under the near-IR lighting source of 760 nm,850 nm,890 nm,960 nm and the combination of every two of them.The ratio of the average gray-scale value of palm vein areas and other areas in the region of interest (ROI) is taken as the criterion of wavelength choice.Palm vein image acquisition with the combination of 760 nm and 960 nm NIR is adopted at last.