Spectroscopy is a new technique to study stem cells.It can provide a certain reference for clinical application.We cultured NSCs and NCs in vitro respectively and achieved their Raman spectra using a laser confocal microscope Raman spectrometer.The results show that there is difference between 646 cm-1,2917 cm-1of NSCs and 652 cm-1,2908 cm-1of NCs.There are more thylakoid of DNA and casein in NCs nuclei while NSCs nuclei has some NO2 which does not exist in NCs.NSCs seems to contain more DNA,tryptophan,adenine,carotenoid,amideⅢ etc than NCs.This paper analyzes difference of Raman spectra of neural stem cells and neural cells in spectroscopy terms in order to provide some experimental reference for clinical application.