The abnormal inner heat source in human body shows a higher surface temperature in corresponding part area of the body.The higher surface temperature can be detected by the Medical Thermography,which is one of the diagnosis methods for the abnormal inner heat source.But the surface temperature distribution which obtained by the Medical Thermography is hard to reflect the correlative information of the abnormal inner heat source directly.So it is necessary to study the relationship between the information of the heat source including the depth,temperature,thermal properties and surface temperature distribution.This study which is based on bio-heat transfer equation builds a finite element analysis model to analyze the influence of the different factors of the inner heat source to the surface temperature distribution of human body.A developed thermostatically but adjustable heat source was buried in the layered biological tissue,and the Medical Thermography was used to record the temperature distribution of each biological tissue layer.Then,the inside and outside temperature distribution was given,and the theory simulation is validated by the physical experiment.The results show that the surface temperature distribution is primarily affected by the depth of the inner heat source,which provides a theoretical and experimental basis for the thermal technology of inner abnormal heat source diagnosis.