The theoretical reconstruction of Laguerre-Gaussian (LG) beams with computer generated holograms (CGHs) is analyzed.LG beams are generated on our holographic optical tweezers platform by loading CGHs into a spatial light modulator (SLM).A series of experiments on trapping and manipulation of absorptive CuO micro-particles are performed.Experiments demonstrate that a single CuO micro-particle can be trapped in the dark central spot of LG beams and rotated around the axis parallel to the optical axis.It can also be trapped in the region of maximum intensity of LG beams and set into rotation around the circumference.In addition,multiple CuO particles can be set into rotation around the circumference.The relationships of the rotation period changing with the laser power and the azimuthal index are obtained by observation and measurement.The results play a guiding role in the accurate manipulation of micro-particles′ rotation.