In order to guarantee the fruit quality and meet consumer and enterprise’s requirements, the quality detection and classification processing of fruit are necessary. Hyperspectral imaging is a non-destructive detection technology with integration of spectroscopy and imaging techniques, which can acquire both spectral and spatial information of fruits rapidly, then it can reflect an overall information of external and internal fruit quality. Therefore, it will have great potential in the non-destructive detecting field of fruit quality. The basic principles, image acquisition and analysis methods of hyperspectral imaging, its applications in domestic and overseas, and the future development are presented.