In order to realize the high resolution scanning for near infrared fluorescence,a laser scanning confocal optical system in the near infrared region was designed.The objective lens was designed with apochromatic microobjective and the point light and lighting lens was designed with concave lens and convex lens,and the emission lens was designed with Coke objective.The software ZEMAX is used for the optical design and simulation.Simulation results show that,when the numerical aperture of the objective lens is 0.42,the focal spot’s size of point light is less than 0.2 μm,and the defocus spot’s diameter is less than 40 μm,which meets the need of the lighting pinhole.The focal spot’s size of lighting lens is less than 1 μm,and the spot’s energy is focused,which is more than 83% in the range 2 μm.The defocus spot’s diameter of emission lens is less than 100 μm,which meets the need of the detecting pinhole.In addition,the lighting lens and the emission lens both have high optical transmission efficiency.The confocal optical system has advantages of high numerical aperture,high resolution and better performance in near infrared region.