A design of OCT endoscopic probe for tissue biopsy is proposed,and this probe can be used for real time imaging of tissue profile based on tissue depth information,so it has broad prospect in clinical application.The design of probe was theoretically analyzed,and then the process and measurement of the probe were elaborated in detail.Compared with the traditional probe,the proposed probe can improve the optical quality and machine robust.The principle that the image quality depends on the distance between single mode fiber and GRIN lens was modeled and verified.The experimental results show that,when the length of glass spacer is about 0.2 mm,the working distance of the probe reaches about 2 cm and its divergence angle of far field is 0.0175°.