In order to verify the rationality and reliability of the theoretical model of modified Friese optical rotation,a real-time and rapid experimental platform of optical tweezers was set up to measure the rotation of uniaxial birefringence particles.The laser microbeam was firstly collected by collecting lens,and then the beam was collected by four-quadrant detector(QD)across dichroic mirror and imaging lens.The signal of four-quadrant detector was collected with data acquisition card.Finally,rotation frequency of sample particles was measured through the analysis of signal and image data,moreover,the analysis and comparisons with theoretical curves were presented in detail.The results show that,the simulation curve of modified Friese model is much consistent with the experimental data.The optical tweezer can be applied to drive micro-nano mechanism,measure mechanical parameters of micro-nano system and assemble biological devices.