The clock bias of navigation satellite is usually millisecond level,and the positioning accuracy of navigation will be affected obviously if the clock bias is not precisely corrected.The principle and calculating method of the BDS satellite clock bias based on SLR and L-band pseudo-range co-location data were described.The clock bias of COMPASS-G1 was computed using the observation data of September 2014 provided by Changchun Observatory.The clock bias is compared with the reference clock bias offered from GFZ.Comparison and analysis results show that the difference of two COMPASS-G1 clock biases is less than 6 ns.It shows that this calculating method of BDS satellite clock bias in this paper is feasible.