The random three-dimensional imaging of fast fluorescence signals in nerve cells has become a hot issue in recent years,which represented a higher requirement for fast axial scanning speed in the two-photon fluorescence microscopy imaging system.Fast axial scanning system based on moving the focus lens group inside objective lens was designed.The focus lens group which is composed of a few lenses near the specimen is driven by the electric displacement actuator,so that the focus of objective lens can be rapidly scanned in the axial direction.The light path between the focus lens group and the main lens group is near parallel,which enables the system to adapt a large scanning range.The weight of the focus lens group is light,which makes the drive load small,so the system scanning speed is much faster.The system was simulated and analyzed by ZEMAX.The results show that the changes of modulation transfer function(MTF) curve are little when the scan range is from 0 to 500 μm.The weight of the focus lens group is only 0.1 g,and the scanning frequency can reach several thousand hertz by using the piezoelectric ceramic as the driving element,which can meet the requirement of large range and fast axial scanning of nerve functional imaging.
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赵绪文,卢景琦.基于移动物镜中少数透镜的快速轴向扫描系统[J].激光与红外,2016,46(11):1379~1383 ZHAO Xu-wen, LU Jing-qi. Fast axial scanning system based on a few lenses in moving objective lens[J]. LASER & INFRARED,2016,46(11):1379~1383