A tunable multi-wavelength Brillouin erbium fiber laser (MWBEFL) with 0.256 nm spacing based on a four-port circulator and two three-port circulators was experimentally proposed.The fiber laser configuration formed by two ring cavities.One cavity consisting of two three-port circulators generated 1st Stokes,and the other cavity consisting of a four-port circulator produced a double Brillouin frequency spacing Stokes signal separated from the injected BP light.The experimental results show that:when BP is 3 dBm and 980 nm pump power is 27.78 dBm,6 output channels with 0.256 nm spacing can be obtained.And the relationship between the number of the Stokes signal and BP power and the 980 nm pump power is discussed.