As an important means of interference,the smoke screen played an important role in countering various types of precision guided weapons. In this paper,based on the influence mechanism of smoke screen interference on the seeker,the influence of the smoke screen on the performance of the infrared seeker is analyzed from four aspects:smoke screen transmittance,smoke screen radiation,smoke screen effective shielding time and target area shielding ratio. Combined with the semi-physical simulation,the quantitative test was carried out,and the scientific and reasonable test data were obtained. The accurate evaluation of the anti-jamming ability of the infrared seeker under the smoke screen interference environment was achieved.