The laser semi active guidance hardware in the loop simulation system based on diffuse reflection screen consists of a diffuse reflection screen,a laser target simulator,2D of target line of sight motion simulated reflectors,a laser jamming simulator,a 2D of jamming line of sight motion simulated reflectors,a 3D of missile flight motion simulator,a simulation computer and a simulation master control system. After the development of each subsystem project is completed,it is necessary to determine the location and direction during installation of each subsystem. In this paper,the main summary of the method of installation,positioning and zero calibration of each part is given,which is a guide for the construction of similar systems.